Rupture probability dependence on average intrinsic curvature. The probability of GUV rupture was examined 30 min after GUVs with 0.5 mol % TR-DHPE were adsorbed to a glass surface. The C′0,ave values of −0.025 (black circle), −0.099 (green circle), −0.11 (red circle), −0.13 (blue circle), −0.15 (orange circle), −0.18 (purple circle), −0.19 (black square), −0.21 (green square), −0.25 (red square), −0.29 (blue square), and −0.31 nm−1 (orange square) correspond to the following 11 lipid compositions, respectively: DOPC/Lyso-PC/DOPG (7:2:1), DOPC/DOPG (9:1), DOPE-Me2/Lyso-PC/DOPG (7:2:1), DOPE-Me2/Lyso-PC/DOPG (7.5:1.5:1), DOPE-Me2/Lyso-PC/DOPG (8:1:1), DOPE-Me2/DOPC/DOPG (7:2:1), DOPE-Me2/DOPC/DOPG (8:1:1), DOPE-Me2/DOPG (9:1), DOPE-Me/Lyso-PC/DOPG (8:1:1), DOPE-Me/DOPC/DOPG (8:1:1), and DOPE-Me/DOPG (9:1). Scale bars show standard errors. N = 17–42.