Figure 2.

Identification of fatty acid elongation activity in yeast expressing a C. elegans ORF. Chromatograms of fatty acid methyl esters from yeast transformed with the control (empty) plasmid pYES2 (A) or with ORF F56H11.4 in pYES2 (B). Exogenous substrate in the form of 18:3, n-6 (annotated as 18:3) was supplied to the cultures. Two additional peaks are observed in B; these peaks were identified (against known standards) as 20:3, n-6 and 18:1, n-7 and are annotated in B as 20:3 and 18:1* (in the latter case, this annotation is to distinguish this 18:1, n-7 fatty acid from 18:1, n-9). Detection was by flame ionization, with peak identification by subsequent GC-MS.