Table 3.
Fatty acid composition of yeast expressing C. elegans ORF F56H11.4 and both fatty acid desaturases required for PUFA biosynthesis
| Fatty acid | mol percentage fatty acids
|
|||
|---|---|---|---|---|
| F56H11.4 + Δ5 + Δ6
| ||||
| 18:2, n-6
|
18:3, n-3
|
|||
| + | − | + | − | |
| 16:0 | 19.1 ± 0.9 | 22.8 ± 1.3 | 17.1 ± 0.9 | 21.8 ± 0.3 |
| 16:1, n-7 | 17.2 ± 0.6 | 23.4 ± 0.3 | 5.2 ± 0.8 | 10.6 ± 0.9 |
| 16:2, n-7 | 0.4 ± 0.2 | — | 0.2 ± 0.1 | — |
| 18:0 | 4.2 ± 0.1 | 5.1 ± 0.1 | 5.6 ± 0.5 | 5.4 ± 0.1 |
| 18:1, n-9 | 12.8 ± 1.5 | 10.5 ± 0.7 | 6.0 ± 0.8 | 6.3 ± 0.6 |
| 18:1, n-7 | 8.0 ± 1.7 | — | 2.5 ± 0.8 | — |
| 18:2, n-6 | 25.8 ± 2.1 | 38.2 ± 1.6 | — | — |
| 18:3, n-3 | — | — | 47.6 ± 3.4 | 55.9 ± 1.4 |
| 18:3, n-6 | 6.8 ± 1.4 | — | — | — |
| 18:4, n-3 | — | — | 10.8 ± 1.9 | — |
| 20:2, n-6 | 3.65 ± 1.2 | — | — | — |
| 20:3, n-6 | 1.4 ± 0.3 | — | — | — |
| 20:3, n-3 | — | — | 3.4 ± 0.2 | — |
| 20:4, n-6 | 0.25 ± 0.1 | — | — | — |
| 20:4, n-3 | — | — | 1.4 ± 0.3 | — |
| 20:5, n-3 | — | — | 0.2 ± 0.1 | — |
| Percentage elongated | ||||
| 18:3, n-6 | 19.5 | — | — | — |
| 18:4, n-3 | — | — | 12.9 | — |
| 18:2, n-6 | 9.6 | — | — | — |
| 18:3, n-3 | — | — | 5.4 | — |
Fatty acid composition of yeast cotransformed with F56H11.4 and both the Δ5- and Δ6-fatty acid desaturases; induction time was 96 h. Either 18:2, n-6 or 18:3, n-3 was supplied as exogenous substrates. Analysis was performed as described for Table 1.