TABLE 1.
Fluorescence emission dynamics of ECFP at different maturational stages of hippocampal neurons
| Cell | DIV | τ1 (ns) | τ1 (%) | τ2 (ns) | τ2 (%) | τ3(ns) | τ3 (%) | τmean(ns) |
|---|---|---|---|---|---|---|---|---|
| Neurons | 7 | 3.39 ± 0.04 | 35 ± 3 | 1.4 ± 0.13 | 35 ± 1 | 0.29 ± 0.04 | 30 ± 4 | 1.76 ± 0.13 |
| 10 | 3.36 ± 0.05 | 44 ± 3 | 1.36 ± 0.07 | 33 ± 1 | 0.19 ± 0.03 | 23 ± 3 | 1.97 ± 0.10 | |
| 15 | 3.45 ± 0.14 | 37 ± 2 | 1.50 ± 0.26 | 34 ± 5 | 0.23 ± 0.14 | 28 ± 3 | 1.96 ± 0.15 | |
| 15 | 3.30 ± 0.02 | 59 ± 1 | 1.08 ± 0.04 | 41 ± 1 | 2.38 ± 0.03 | |||
| COS-7 | 3.26 ± 0.05 | 57 ± 1 | 1.06 ± 0.03 | 43 ± 1 | 2.31 ± 0.05 |
The multiple lifetimes and individual contributions of ECFP are shown at different stages of neuronal development. The percentage of contributions of τ1, τ2, and τ3 are denoted as τ1%, τ2%, and τ3%, respectively. The major fraction of cells at DIV 15 (60%) displayed biexponential decay for ECFP in contrast to young stages. COS-7 cells showed lifetimes and contributions similar to the majority of mature neurons.