TABLE 2.
Fluorescence emission dynamics of Clomeleon at different stages of hippocampal neuronal development
| Cell | DIV | N(%) | Class | R | τ1 (ns) | τ1 (%) | τ2 (ns) | τ2 (%) | τ3 (ns) | τ3 (%) | τmean (ns) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Neurons | 7 | I | 0.42 ± 0.04 | 3.39 ± 0.05 | 40 ± 2 | 1.35 ± 0.06 | 33 ± 1 | 0.26 ± 0.03 | 27 ± 2 | 1.86 ± 0.07 | |
| 10 | 58 | I | 0.47 ± 0.06 | 3.37 ± 0.06 | 45 ± 3 | 1.38 ± 0.13 | 32 ± 3 | 0.24 ± 0.10 | 23 ± 2 | 2 ± 0.10 | |
| 10 | 33 | II | 1.07 ± 0.08 | 34 ± 1 | 33 ± 2 | 33 ± 2 | 1.68 ± 0.04 | ||||
| 14 | 40 | I | 0.65 ± 0.07 | 3.42 ± 0.05 | 39 ± 4 | 1.36 ± 0.07 | 32 ± 2 | 0.22 ± 0.05 | 29 ± 5 | 1.84 ± 0.15 | |
| 14 | 40 | II | 1.03 ± 0.11 | 35 ± 2 | 35 ± 2 | 31 ± 2 | 1.73 ± 0.08 | ||||
| 14 | 20 | III | 1.23 ± 0.04 | 30 ± 1 | 34 ± 2 | 35 ± 2 | 1.57 ± 0.01 | ||||
| COS-7 | 1.05 ± 0.18 | 3.40 ± 0.02 | 32 ± 2 | 1.32 ± 0.05 | 36 ± 1 | 0.17 ± 0.01 | 32 ± 2 | 1.61 ± 0.06 |
The lifetimes of Clomeleon were consistent along development, though a difference in the contributions of the individual lifetimes was observed. The percentage of the total population of cells in each class is shown (N%). The percentage of contributions of τ1, τ2, and τ3 are denoted as τ1%, τ2%, and τ3%, respectively. The mean lifetimes for the respective classes at each stage are denoted as τmean. Comparison of lifetimes and contributions obtained from COS-7 cells showed that they were similar to class III at DIV 14.