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. Author manuscript; available in PMC: 2008 Jan 1.
Published in final edited form as: Arch Oral Biol. 2006 Oct 12;52(1):90–99. doi: 10.1016/j.archoralbio.2006.07.003

FIGURE 1.

FIGURE 1

A sampling frame was created by assigning a unique number to each isolate and then, within each phenotypes, 12 isolates were randomly chosen using a computer generated random numbers list. Wells of microtiter plates were coated with washed whole cells of the selected isolates and an ELISA was performed as described in Materials and Methods using serial twofold dilutions (1:500 to 1:32,000) of rabbit antisera raised against S. oralis, SK100 and S. mitis biovar 1, SK145. Individual plates were used for each antiserum which always included the homologous strain. Pre-immune serum served to control for natural antibodies reactive with the antigen. For each isolate tested the sum of the absorbance value at each dilution was expressed as a percentage of the sum of the absorbance value at each dilution of the homologous strain. Filled bars represent SK145 antiserum and open bars represent SK100 antiserum. The interrupted horizontal lines are set at 25%, 50%, and 75% of the antibody binding of the homologous control. In the text isolates of the respective phenotypes are assigned the prefix A, B, C or D.