. Author manuscript; available in PMC: 2008 Feb 1.

Published in final edited form as: Toxicol In Vitro. 2006 Sep 6;21(1):125–132. doi: 10.1016/j.tiv.2006.08.004

Table 1.

LDH release and Total LDH Activity in LLC-PK1 cells incubated with para-aminophenol.

[PAP] (mM)	NADH oxidation		Resorufin formation
[PAP] (mM)	LDH release (% of total)	Total LDH (% control)	LDH release (% of total )	Total LDH (% control)
0	4.6 ± 4.6	100 ± 0	7.9 ± 2.3	100 ± 0
0.1	7.9 ± 4.8	101.9 ± 3.0	4.5 ± 0.9	97.6 ± 4.3
0.5	6.9 ± 3.5	83.0 ± 2.6^*	2.9 ± 1.6	71.2 ± 8.5^*
1	7.0 ± 2.8	85.0 ± 6.7^*	2.0 ± 1.0	65.1 ± 8.9^*
5	2.7 ± 2.7	76.0 ± 2.3^*	1.9 ± 1.0	67.7 ± 6.8^*
10	0 ± 0	65.8 ± 5.4^*	2.6 ± 1.5	67.7 ± 5.4^*

Cells were incubated for 4 h with PAP (0–10 mM) dissolved in HBSS. For NADH oxidation, cells were plated in 60 mm dishes and LDH was measured as the change in absorbance at 340 nm. For resorufin formation, cells were incubated in 24-well plates and LDH activity was determined using the CytoTox-One assay protocol. Data are expressed as means ± SE of 3 independent experiments.

Significantly different from control (0 mM PAP) by one-way ANOVA with SNK post-hoc test (p < 0.05).