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Salmonella Strain Dependent Expression of Flagellin and NF-κB Activation
| NF-κB Activation (infected/uninfected) |
|||
|---|---|---|---|
| Strain | Flagellin | Observed | Theoretical1 |
| fljB−/fliC+ | 15.5 | 22 | 174 |
| fljB+/fliC− | 0.11 | 1 | 1 |
| fljB+/fliC+ | 26.1 | 37 | 237 |
Theoretical induction activity was calculated from the amount (ng) of flagellin protein detected in the inoculum bacteria (7.5 x 106 CPU) compared to the activity observed following treatment of HEK293-Luc cells with varying amounts of purified monomeric flagellin.
