Supernatants from treated BMDC were harvested at 24 hours and analyzed for the presence of inflammatory cytokines. A, Levels of TNFα, MCP-1, and IL-6 in BMDC supernatants as measured by CBA (see Materials and Methods). B, IL-12p40 levels in BMDC supernatants were measured by ELISA. C, Type I IFN levels in BMDC supernatants relative to mock treated BMDC as measured by the VSV-based IFN-α/ß bioassay. Levels of IFNα/β in polyI:C treated BMDC supernatants were similar to LPS. * P< .05 compared with mock-infected BMDC (A and B) or compared with VV-infected BMDC (C). Values are an average of 3 experiments.