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. Author manuscript; available in PMC: 2008 Mar 15.
Published in final edited form as: Virology. 2006 Oct 31;359(2):283–294. doi: 10.1016/j.virol.2006.09.034

Figure 8. Analysis of fetuin B and fetuin A mRNA expression in liver and HCC.

Figure 8

A) Northern blot assay for fetuin B and fetuin A mRNA transcripts in HCC and adjacent non-cancerous liver from woodchucks chronically infected with WHV. Each lane contained 5μg total RNA; 18S ribosomal RNA was visualized as a control. B) Semi-quantitative RT-PCR analysis for fetuin B and fetuin A mRNA expression in human HCC and non-cancerous surrounding liver from 3 individuals (patient 7, patient 8 and patient 9) chronically infected with HBV. After reverse transcription, the cDNA was diluted 10-fold and 100-fold and used as a template for PCR. β-actin was used as an internal control. Human fetuin B mRNA transcripts were at least 10-fold lower in the HCC compared to surrounding liver. Fetuin A mRNA expression was unchanged.