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Induction of p21 and p53 by TNP-470. HUVECs were synchronized and treated as in Fig. 1. (A) HUVEC lysates were subjected to Western blot analysis to determine the expression levels of p21, p27, p53, and actin. (B) TNP-470 treatment increases the levels of p21 associated with the cyclin E-Cdk2 complex. The cyclin E-Cdk2 complex was immunoprecipitated from lysates by using an anti-cyclin E Ab, and the bound p21 and Cdk2 was detected by Western blot analysis.
