Figure 2.

Nucleotide and amino acid sequences of the 5′ region encompassing exon 1b. (A) Sequence surrounding the Cα1b exon. Numbering is relative to the transcriptional start site (+1). The NcoI restriction site is boxed. The sequence matching the degenerate primer Cα12 is underlined. The splice donor site is in bold and double underlined. (B) Comparison of the 3′ end of the mouse exon 1b (mCα2) and human exon 1b (hCα2), a part of the 5′ end region of mouse pseudogene Cx (mCx), a part of the 5′ end of the human Cγ retrogene (hCγ), the 3′ end of exon 1a of the Cα gene (mCα1), and 5′ sequences of C. elegans exons N′2 and N′6 (see Results). Dots indicate gaps introduced in the sequence for alignment purposes. The position of the initiating methionine (Met) is indicated. (C) Comparison of the deduced peptide sequence of mouse and human exon 1b, the deduced amino acid sequences of N′2 and N′6 (C. elegans), and the amino-terminal sequences of Cs and the mouse Cα1. Conserved amino acids are boxed.