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Western blot analysis of testis. Protein (5 μg) from mouse heart, brain, sperm, and P10, P19, or P42 testis was separated by SDS/PAGE (10%), transferred to membrane, and probed with Cα antiserum, showing the somatic catalytic isoform with a molecular mass of 40 kDa in heart and in testis and a smaller subunit in testis at P42. The antiserum also reveals a smaller catalytic isoform in sperm in comparison to the somatic 40-kDa C isoform in brain.
