Figure 2.

Effect of Abl SH3 domain on Cbl phosphorylation. (A) The binding of various SH3 domains to Cbl. Various GST-fused SH3 domains, as indicated at the top, were prepared from bacteria and incubated with lysates from 293T cells expressing Cbl. GST-fusion proteins were immunoprecipitated with glutathione beads and Western-blotted with anti-Cbl antibody. (B) Role of the SH3 domain of Abl for the Cbl phosphorylation. Proteins (50 μg) from total lysates (TL) of 293T cells transfected with the indicated plasmids were resolved by SDS/PAGE and Western blotting with anti-phosphotyrosine (pY) antibody. Arrows indicate the position of Abl, Cbl, and Src.