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Abl and Cbl interact through their C-terminal regions. Proteins (50 μg) from total lysates (TL) of 293T cells transfected with the indicated plasmids were resolved by SDS/PAGE and Western-blotted with anti-FLAG tag (FLAG) antibody recognizing the FLAG-tagged Abl proteins. GST-Cbl fusion proteins were immunoprecipitated (IPGST) with glutathione beads and Western-blotted with the indicated antibodies.
