Figure 3.

Binding of bis-ANS to the pressure-dissociated/denatured TTR at different pHs. Bis-ANS (10 μM) was added to each sample containing 1 μM TTR. The spectral area (A) of bis-ANS emission measured after each increase in pressure was divided by the initial spectral area (A₀) at atmospheric pressure. The samples were pressurized at pH 7.5 (squares), pH 5.6 (circles), or pH 5 (triangles). The decompression pathway is represented by empty symbols. All experiments were performed at 37°C and excitation was set at 360 nm and emission collected from 400–600 nm. (Inset) Shift in the center of mass of the bis-ANS fluorescence emission induced by pressure at pH 5.6, 37°C.