Table 1.
Bacterial strains and plasmids used in this study
Strain or plasmid | Genotype or relevant characteristic | Reference or source |
---|---|---|
E. coli | ||
XL1-Blue | RecA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, lac, [F′, proAB, lacIqZΔM15, Tn10 (tetr)] | 20, Stratagene |
M15(pREP4) | Lac, ara, gal, mtl, recA+, uvr+, [pREP4, lacI, kanr] | 21 |
Plasmids | ||
pQE30 | High copy His-tag expression vector | Qiagen |
pNCO113 | High copy expression vector | ATTC, PTA-852 |
pQEygbParakom | This study | |
pNCOygbPara | This study |