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Purification of recombinant 4-diphosphocytidyl-2C-methyl-d-erythritol synthase from A. thaliana (pseudomaturated protein comprising residues 76–302)
| Procedure | Total protein, mg | Specific activity, μmol⋅min−1· mg−1 | Total activity, μmol⋅min−1 | Yield, % | Purification factor |
|---|---|---|---|---|---|
| Cell extract | 128 | 33 | 4,224 | 100 | 1 |
| Source 15 Q | 17.5 | 67 | 1,172 | 28 | 2 |
| Superdex G 200 | 9.5 | 67 | 637 | 15 | 2 |
