Figure 4.

Cdc13 protein forms multiple complexes with DNA. (A) 150 nM 34-kDa recombinant Cdc13 DBD incubated with differing amounts of unlabeled yeast d(TGTGTGGG)₃ oligomer (1.5-fold dilutions ranging from 24 to 620 nM), plus 1 nM 5′ end-labeled yeast d(TGTGTGGG)₃ oligomer. The two complexes are indicated by arrows. (B) 150 nM 34-kDa recombinant Cdc13 DBD incubated with differing amounts of unlabeled d(TG)₁₈ oligomer (25 nM, 50 nM, 100 nM, 250 nM, 500 nM, and 1,000 nM, from left to right), plus 1 nM 5′ end-labeled d(TG)₁₈ oligomer. (C) The 28-kDa Cdc13-DBD (lane 2), the 34-kDa Cdc13-DBD (lane 3), or an equal mixture of each (lane 1) was incubated with 6 μM unlabeled d(TG)₁₈ oligomer, plus 1 nM 5′ end-labeled yeast d(TG)₁₈ oligomer; proteins, 5 μM. Note that, in the left lane, the mixed complex II band is expected to be present at a 2-fold higher ratio relative to complex II bands that result from solely the 28-kDa protein or the 34-kDa protein.