Table 3.
Expression levels NPY, AgRP, POMC, and CART mRNAs
| mRNA
|
wild type
|
Y4−/−
|
ob/ob
|
Y4−/−, ob/ob
|
|---|---|---|---|---|
| Neurons in the arcuate nucleus | ||||
| NPY | 100 ± 5.48 (5) | 98 ± 3.22 (5) | 178 ± 7.44 (5)c | 155 ± 0.57 (3)cd |
| AgRP | 100 ± 4.70 (4) | 95 ± 3.65 (5) | 167 ± 7.28 (5)c | 136 ± 8.38 (3)be |
| POMC | 100 ± 3.91 (5) | 99 ± 4.84 (4) | 33 ± 3.00 (5)c | 39 ± 0.45 (3)c |
| CART | 100 ± 2.21 (5) | 103 ± 2.67 (5) | 23 ± 1.26 (5)c | 26 ± 3.27 (4)c |
| GnRHI neurons in the forebrain | ||||
| Med. Sept. n. | 100 ± 6.63 (5) | 123 ± 2.10 (5)a | 104 ± 4.33 (5) | 122 ± 8.81 (4)ad |
| Preopt. a. | 100 ± 6.42 (5) | 120 ± 4.33 (5)a | 103 ± 4.62 (5) | 120 ± 3.85 (4)ad |
| Neurons in the cortex | ||||
| NPY | 100 ± 4.20 (5) | 98 ± 5.29 (5) | 98 ± 6.04 (5) | 97 ± 7.72 (4) |
| Neurons in the paraventricular nucleus | ||||
| CART | 100 ± 3.36 (4) | 102 ± 4.24 (5) | 96 ± 2.38 (5) | 101 ± 3.10 (3) |
Data represent mean labeling intensity of neurons given as percentage of wild type ± SEM (number of animals); aP < 0.05, bP < 0.01 and cP < 0.001 versus wild-type; dP < 0.05 and eP < 0.01 versus ob/ob. Med. Sept. n. = medial septal nucleus; Preopt. a. = preoptic area.