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. 2002 May 1;16(9):1089–1101. doi: 10.1101/gad.988402

Figure 5.

Figure 5

Molecular analysis of altered limb development of Dlx5/6−/− embryos. Whole-mount in situ hybridization shows that Shh and Lmx1b expression is normal at E10.5 and E11.5 in the Dlx5/6 null embryos. Fgf8 and Msx2 expression in the hindlimb AER is normal at E10.5, but is absent in the medial AER by E11.5. Arrowheads in the right-hand column indicate the loss of expression and missing tissue in the medial portion of the distal limb in the Dlx5/6−/− embryos. Analysis with a Dlx5 5′ riboprobe for the Dlx5 exon still present in the mutant allele reveals diminished Dlx5 expression in the medial AER by E11.5. Immunohistochemical staining and quantitation of BrdU incorporation reveals decreased cellular proliferation in the AER at E10.5 and E11.5, while proliferation in the underlying mesenchyme is unaffected. A compensatory increase of Dlx2 expression in the AER was not observed.