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. 2002 May 1;16(9):1139–1149. doi: 10.1101/gad.222702

Figure 3.

Figure 3

Repressor activity of WRKY6. (A) WRKY6 promoter-driven GUS reporter gene activity (6p) was monitored in wild-type plants, in the knockout mutants wrky6-1 and wrky6-2, as well as in the overexpressor line CaMV 35S::WRKY6-9 (-9). Shown are GUS-stained roots (R), senescing leaves (SL), and mature leaves 5 h postinoculation with 108-CFU bacterial solution (+Ps avrRPM1) or with 10 mM MgCl2 (control). (B) Transient cotransfection assays with different target gene promoters and WRKY6. Presented are relative activities of WRKY6 promoter (6p), WRKY42 promoter (42p), tetramerized W2-box (4xW2), and mutated tetramerized W2-box (4xmW2) driven GUS reporter gene constructs after transfection of cell culture-derived Arabidopsis protoplasts. Transient transfections were done either with reporter constructs alone or combined with an effector construct containing a CaMV 35S-driven WRKY6 cDNA (WRKY6) or a CaMV 35S-driven fusion of the WRKY6 cDNA to the VP16 activation domain (WRKY6–VP16). Each bar represents the median of four independent transfections. Normalized GUS values were obtained using a control luciferase plasmid for standardization. Relative fold induction or repression values ⩾twofold are depicted.