Table 1.
cDNA-AFLP fragments and homologies to sequences in the Arabidopsis database
Clonea
|
Expressionb
|
Accession no.c
|
Similarity
|
W
|
as1d
|
---|---|---|---|---|---|
R11e | +++ | AC024081 | JA-regulatory protein NAC2 | 2 | 0 |
R16 | ++ | AC011698 | NAM-like protein | 2 | 0 |
R18 | −− | T06055 | kinesin domain containing protein | 4 | 2 |
R40 | ++ | AB023034 | xylosidase | 2 | 1 |
R41e | −− | AC010718 | putative calmodulin | 5 | 1 |
R43 | −− | AB003590 | sulfate transporter | 2 | 1 |
R48 | +++ | AJ270302 | putative β-galactosidase | 3 | 0 |
R50 | −− | AC010926 | putative casein kinase | 3 | 1 |
R52/53 | −− | AL163818 | Ca2+-transporting ATPase-like protein | 4 | 2 |
R62e | +++ | S66346 | SEN1 | 5 | 1 |
R64 | +++ | X89866 | glutathione peroxidase | 0 | 0 |
R67e | −−− | AC006551 | alcohol dehydrogenase-like | 2 | 0 |
R68 | −−− | T02156 | glucosidase homolog | 4 | 1 |
R72 | −−− | AC007651 | putative glutathione transferase | 3 | 1 |
R74e | −− | T05493 | thaumatin-like PR protein | 3 | 0 |
R81 | −−− | AF058919 | putative calmodulin-binding heat shock protein | 3 | 1 |
R92 | −− | AC000132 | receptor-like protein kinase | 2 | 2 |
R96 | −− | AC011708 | putative pectin esterase | 4 | 1 |
R102e | +++ | AC006931 | putative lipase | 0 | 1 |
R105 | − | AF217546 | calmodulin-binding protein | 1 | 0 |
R129 | ++ | AC009519 | MAP kinase-like protein | 1 | 2 |
R140e | +++ | 2924653 | heat shock protein HSP81-2 | 4 | 0 |
R143 | + | AC020579 | putative disease resistance protein | 5 | 3 |
R144e | ++ | Y14590 | class IV chitinase | 6 | 1 |
P2 | +++ | AC005275 | putative xyloglucan endotransglycosylase | 1 | 2 |
P4 | +++ | AL162506 | fructose-bisphosphate aldolase-like protein | 5 | 1 |
P7e | −− | AC002339 | zinc protease-like protein | 2 | 2 |
P24e | +++ | T00540 | receptor-like protein kinase | 9 | 0 |
P27e | +++ | AC002336 | putative expansin | 3 | 0 |
P29 | − | S51478 | drought-induced protein Di19 | 1 | 0 |
P34e | ++ | AC005724 | putative calmodulin-binding protein | 2 | 2 |
P35 | ++ | AL163812 | fructosidase-like protein | 2 | 0 |
P38 | −−− | T09930 | thioredoxin homolog | 0 | 0 |
P40 | − | T04549 | AP2 domain protein homolog | 2 | 0 |
P55e | −−− | AC010676 | putative porin | 2 | 0 |
P57 | − | AC006592 | homeobox factor HB6 | 2 | 0 |
P58 | − | T02644 | ABC-type transport protein homolog | 2 | 1 |
Designated cDNA-AFLP fragments.
Differential expression of the cDNA-AFLP fragments detected in the null-mutant or the overexpression line compared to wild type.
GenBank accession numbers of identified genes corresponding to the cDNA-AFLP fragments.
Number of W boxes and as1-like elements present within 1 kb putative promoter sequence of corresponding genes.
Confirmed by RNA blot or RT–PCR analysis (others not tested).