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. 2002 May 1;16(9):1150–1162. doi: 10.1101/gad.219302

Figure 3.

Figure 3

Ectopic Ubx expression from 24–22 h BPF can suppress sternopleural bristle precursor development on T2, but ectopic expression from shortly before puparium formation cannot. Larvae were subjected to heatshock regimes covering different spans of third larval instar development. Larvae were examined for sensory organ precursors at a stage corresponding to the white prepupa. Precursors were visualized by staining with anti-β-galactosidase in the neuralized-lacZ background (red). Ubx levels were assayed by costaining for Ubx protein (green). (A) Summary table. (B–C) Individual for which the heatshock-Ubx regime started between 24 and 22 h BPF. (B) Second leg disc of this individual. No sternopleural bristle precursor is found in the dorsal leg periphery. (C) Third leg disc of this individual. (D–E) Individual for which the hs-Ubx regime started 7 h BPF. (D) Second leg discs of this animal. The sternopleural bristle precursor is present in the dorsal leg periphery (arrowhead). (E) Third leg of this animal. (C,E) In both treatments, ectopic Ubx levels at the time of collection have not sunk below endogenous third leg levels.