Figure 1.

Separation of D. punctata DH performed by RP-HPLC (5-μm Vydac 300-Å C₁₈ column, 4.6 mm × 150 mm, eluted at 1.0 ml/min) with a gradient of aqueous acetonitrile (composition indicated by dashed line) with constant 0.1% (vol/vol) heptafluorobutyric acid. Two-milliliter fractions were collected; those at 32–34 min (A) and 54–56 min (B) had biological activity. The BSA added to the extraction solvent eluted as a broad peak (arrow).