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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Regul Pept. 2006 Dec 13;139(1-3):109–114. doi: 10.1016/j.regpep.2006.10.013

Figure 4. Activation of c-Src by m2 receptors.

Figure 4

Gastric muscle cells were treated with ACh (0.1μM) in the presence or absence of methoctramine (0.1 μM) or PP2 (1 μM) for 5 min and activation of c-Src (p-c-Src) was measured using phospho-Src (Tyr527) antibody. Pertussis toxin (400 ng/ml) was added for 60 min and then stimulated with ACh. Immunoblot analysis showed equal amounts of loaded protein (not shown). Values are expressed as means ± SE of four experiments. ** P<0.001 significant increase in c-Src activation by ACh.