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. 2002 May 15;16(10):1291–1302. doi: 10.1101/gad.981802

Figure 4.

Figure 4

PRY-1 is a functional Axin homolog. (A) PRY-1 inhibits the Wnt-1-dependent activation of a TCF reporter gene in 293-T cells. (Black bars) Activation of the Tcf reporter TOP; (gray bars) activation of the negative control FOP. (B) SGG-1 also inhibits the Wnt-1-dependent activation of the Tcf reporter. Expression of PRY-1 resulted in a phenotypic rescue of the optic and anterior brain structure defects of (D) zebrafish mbl/axin1 mutants that was indistinguishable from (C) wild-type embryos (see Table 3). Overexpression (XS) in wild-type zebrafish embryos of (E) Axin1 or (F) PRY-1 induced identical phenotypes, such as deletion of the trunk posterior to the otic vesicle. Overexpression of PRY-1 and Axin1 was carried out by microinjection of 25 or 150 pg of the respective mRNAs into one-cell stage zebrafish embryos.