Table 1.
Genetic interactions between pry-l and components of theegl-20/Wnt pathway
| Genotype
|
%
QL descendants in posterior
|
% QR descendants in
posterior
|
|---|---|---|
| Wild type | 100 | 0 |
| pry-l(RNAi) | 100 | 48 |
| egl-20(n585) | 0 | 0 |
| egl-20(n585); pry-l (RNAi) | 82 | 34 |
| bar-1(ga80) | 1 | 0 |
| bar-1(ga80); pry-l(RNAi) | 0 | 0 |
| pop-1(hu9) | 91 | 0 |
| pop-1(hu9); pry-l(RNAi) | 100 | 2 |
| mab-5(el239) | 0 | 0 |
| mab-5(el239); pry-l (RNAi) | 6 | 0 |
| mig-5(RNAi) | 21 | 0 |
| pry-l(mu38) | 100 | 55 |
| pry-l(mu38); mig-5(RNAi) | 100 | 46 |
The RNA interference experiment was performed by feeding animals on E. coli expressing double-stranded RNA of pry-l, mig-5, or the empty vector L4440 as described (Kamath et al. 2001; Timmons et al. 2001). In each case, n = 100. The final positions of QL.paa (PVM) and QR.paa (AVM) were scored in L4 larvae using a mec-7::gfp reporter transgene. Final position was scored as anterior or posterior to the vulva.