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A single pulse of activated BAR-1 in the late L2 is sufficient to cause an Overinduced phenotype. Partially synchronized L1 larvae of the genotype huIs1 (○) or bar-1(ga80); huIs1 (♦) were grown at 20°C and given a single heat shock at the indicated time after placing them on plates with E. coli. The corresponding larval stages are indicated. The percentage of animals displaying an Overinduced phenotype is plotted for each time point.
