Figure 2.

Binding of in vitro translated DP1/E2Fs to different E2F elements is regulated by CpG methylation. DP1 and either E2F1, E2F2, E2F3, E2F4, or E2F5 were co-translated in vitro, and the interaction of the resulting heterodimers with the E2Fdhfr, E2F_E2F1-A, E2F_E2F1-B, E2Fcdc2, E2Fc-myb, and E2Fc-myc radiolabeled probes was analyzed by EMSA. Before labeling, the DNA probes were either methylated with Sss I (+) or control methylated (−). No cDNA was added to the control (CNTL) in vitro transcription-translation reaction. Only the retarded E2F/DNA complexes are shown.