Figure 2.

Inhibitory activities of compound 12 against FIV (A), SIV (B), and HIV (C) in tissue culture. (A) Amount of FIV was determined by counting pelletable FIV reverse transcriptase in culture supernatant. (B) The effectiveness of the inhibitor against SIV was determined by using a quantitative ELISA assay by measuring OD at 405 nm at 4 days postinfection. (C) The level of efficacy of inhibition of HIV replication was assessed by measuring viable cells in cultures. Against all three virus, compound 12 showed almost the same degree of potency in tissue cultures and became most effective at 1.0 μg/ml (1.1 μM) to block nearly 100% of viral reproduction.