Figure 4.

Hox11 triple mutant blastemas undergo apoptosis in the absence of induction. Consistent with loss of metanephric induction, the condensed blastemal mesenchyme is lost by apoptosis in the triple mutant. (A) Control and (B) Hox11 triple mutant, show sections through E11.5 embryos. Cell death staining by TUNEL is shown in green, and Pax2 antibody staining is in red. Note massive cell death in the triple mutant blastema, but not the Wolffian ducts. The metanephric blastemas in control and mutant embryos are indicated by a white arrow; the Wolffian ducts are indicated by a white W.