Figure 3.

Expression of SF1 and Scc in 13.5 dpc Dhh^+/− and Dhh^−/− XY gonads. (A) Colocalization of SF1 (green nuclear staining) and Scc (red cytoplasmic staining) in Leydig cells in normal 13.5 dpc XY gonads by immunocytochemistry for SF1 and in situ hybridization for Scc. (B) Strong nuclear SF1 staining (arrows) in Leydig cells in Dhh^+/− gonads. (C) Absence of strong nuclear SF1 staining in Leydig cells in Dhh^−/− gonads. Weak nuclear SF1 staining was still present (arrowheads). SF1 staining was also detected in Sertoli cells (asterisks) in testis cords (TC, outlined by dotted lines). Germ cells and endothelial cells were stained with an anti-PECAM antibody (blue staining in B and C).