Figure 4.

DHH/PTCH1 signaling is not responsible for induction of mesonephric cell migration into XY gonads. (A) Analysis of Ptch^LacZ expression in 12.0 and 12.25 dpc XY gonads. (B) Recombinant organ culture using a wild-type gonad apposed to a Ptch^LacZ mesonephros. (C) Reciprocal organ culture with a Ptch^LacZ gonad apposed to a wild-type mesonephros. Recombinant organ cultures in both B and C were assembled at 11.25 dpc, cultured for 48 h, and assayed for Ptch^LacZ expression. (D) Mesonephric cell migration and Scc expression in Dhh^+/− and Dhh^−/− gonads: Recombinant gonad cultures were assembled with an 11.5 dpc Dhh^+/− or Dhh^−/− XY gonad apposed to an 11.5 dpc mesonephros expressing GFP. Cell migration (green cell, red arrows) was detected 48 h after culture. Samples were fixed, and then expression of Scc was detected by in situ hybridization (red staining in gonads).