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Effects of the hedgehog inhibitor cyclopamine on proliferation and apoptosis in 11.5 dpc gonads. Gonads (11.5 dpc) were cultured for 24 h in the presence or absence of cyclopamine (25 μM) followed by immunocytochemistry for phosphorylated Histone H3 (arrows) or LysoTracker staining for apoptosis (arrows indicate position of the Müllerian duct). G, gonad (outlined by a dotted line); M, mesonephros.
