Table 1.
Strains used in this study
| Strain
|
Genotype
|
Source
|
|---|---|---|
| YMP10860a | MATαRNR1-LEU2 sml1d::KAN his3 leu2 trp1 ura3 | L. Hartwell |
| 12511-2-2ba | Same as YMP10860b but mec1Δ::TRP1 | L. Hartwell |
| Y300 | MATa can1-100 ade2 his3 leu2 trp1 ura3 | S. Elledge and Y. Sanchez |
| Y801 | Same as Y300 but chk1Δ::HIS3 | S. Elledge and Y. Sanchez |
| PKY 238 | MATa CDC28-HA::URA3 leu2 trp1 his3 ade2 | M. Solomon and P. Kaldis |
| OCF 1522 | MATa bar1 PDS1-HA::URA3 leu2 trp1 his3 ade2ura3 | (Cohen-Fix and Koshland 1997) |
| OCF 1525 | Same as 1522 but pds1Δ::LEU2 | (Cohen-Fix and Koshland 1997) |
| RA2817-1d | MATa cdc28-13 ESP1-Myc::TRP1 ura3 leu2 his3 | This study |
| RA2817-8a | MATa ESP1-Myc::TRP1 ura3 leu2 his3 | This study |
| cdc28-as1 | MATa bar1 cdc28d::cdc28-as1 ura3 trp1 ade2 can1-100 | David Morgan and Jeff Ubersax |
| RA2808 | Same as 1522 but pds1T304A-HA::URA3 | This study |
| RA2810 | Same as 1522 but pds1S277AHA::URA3 | This study |
| RA2811 | Same as 1522 but pds1S292A-HA::URA3 | This study |
| RA2815 | Same as 1522 pds1S277AS292T301A-HA::URA3 (also referred to as pds1-38) | This study |
| OCF1548-6D | MATa bar1 ESP1-Myc::TRP1 his3 leu2 ura3 ade2 | This study |
| OCF1548-5C | MATa PDS1-HA::URA3 ESP1-Myc::TRP1 his3 leu2 ade2 | This study |
| RA2806-2b | MATa pds1-38-HA::URA3 ESP1-Myc::TRP1 leu2 his3 ade2 | This study |
| RA2804-25c | MATa pds1Δ::LEU2 ESP1-Myc::TRP1 ura3 his3 ade2 | This study |
a
A derivative of the A364a background.