Expression analysis of XWBSCR11 during Xenopus development. (A) RT–PCR analysis with RNA isolated from whole embryos demonstrates the expression of XWBSCR11 throughout Xenopus embryogenesis. (B) RT–PCR of tissue explants from blastula (stage 8/9) and early gastrula (stage 10) embryos. The schematic diagram shows the location of the dissection for the various tissue explants. XWBSCR11 does not appear to be differentially expressed between animal cap (AC), marginal zone (MZ), or vegetal pole (VG) tissues in blastula and gastrula embryos, nor between dorsal (DMZ) and ventral marginal zone (VMZ) in gastrula embryos. RT–PCR of histone H3 is shown as a loading control in both A and B. (C) Whole mount in situ analysis of XWBSCR11 expression. (a) Gastrula stage; (b–g) neurula stages; (h–i) tailbud stages. In a–c, e, and g–i, anterior is left.