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. 2002 Jun 15;16(12):1518–1527. doi: 10.1101/gad.1001502

Figure 2.

Figure 2

Figure 2

Loss of Dot1 abolishes histone H3 Lys 79 methylation in vivo. (A) Western blots of yeast whole-cell extracts and recombinant yeast histone H3 were probed with either antibodies against the histone H3 tail (residues 1–20) or against a peptide containing methylated Lys 79. (B) Specificity of the anti-methyl Lys 79 antibody. Antibodies (1/10,000 dilution) were preincubated with either no peptide, 25 μg nonmethylated peptide, or 25 μg of methylated Lys 79 peptide for 30 min at room temperature before probing yeast whole-cell extracts for histone H3. (C) Western blot screen for histone H3 Lys 79 methylase. Whole-cell extracts from the indicated deletion strains were probed with antibodies against methylated Lys 4, methylated Lys 79, and unmodified H3. (D) Restoration of Lys 79 methylation by constructs containing the DOT1 gene. Whole-cell extracts from the indicated strains were probed with either anti-methyl Lys 4, or anti-methyl Lys 79 antibodies.