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. 2002 Jun 15;16(12):1518–1527. doi: 10.1101/gad.1001502

Figure 4.

Figure 4

Dot1 methylation of Lys 79 of histone H3 is required for telomeric silencing. (A) URA3-based telomeric silencing assay in which equal numbers of wild-type (WT) and mutant (dot1, K79A, K79P, K79Q) strains were spotted at 10-fold dilution on synthetic complete (SC) medium in the presence or absence of 0.12% 5-fluoro-orotic acid (FOA) and/or Uracil (Ura), and incubated at 30°C for 3 d. (B) ADE2-based telomeric silencing assay in which the indicated wild-type and mutant strains were patched on YPD medium with or without 40 mg/L adenine for 2 d and transferred to 4°C for 2 d. These strains (WZY42 background) contain an ADE2 reporter integrated adjacent to the telomere of chromosome V.