Table 3.
lin-35 | rsr-2 | lsm-2 | lsm-4 | snr's (1–7) | |
RNAi phenotype in WT | Rbs | Ste, Lva | WT | WT | Emb, Lva, Lvl |
synMuv B | +++ | + | + | + | + |
synMuv B (25°C) | +++ | n/a | ++ | ++ | n/a |
Transgene silencing (25°C) | ++ | - | - | - | n/a |
Ectopic lag-2::GFP | + | - | - | - | + |
Ectopic lag-2::GFP (25°C) | ++ | - | ++ | ++ | + |
Extra intestinal cells | + | + | - | - | - |
Ectopic P granules | +++ | - | - | - | + |
cyd-1 rescue | + | - | - | - | - |
Summary of RNAi phenotypes of splicing related genes: synMuv B activity, transgene silencing (scm::GFP), ectopic expression of lag-2::GFP reporter, extra intestinal cells (elt-2::GFP), ectopic P granules, and rescue of cyd-1. If 25°C is not indicated, experiments were performed at 20°C. “+++”, “++”, “+” and “–“stand for strong, moderate, low and no effect respectively. (n/a: not annotated).