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. 2007 Apr 25;104(18):7444–7448. doi: 10.1073/pnas.0702136104

Fig. 3.

Fig. 3.

QS11 synergizes with Wnt-3a in activating Wnt signaling through ARF activation. (A) QS11 increased cellular ARF-GTP levels. NIH 3T3 cells were treated with QS11, DMSO, or QS11-NC at the indicated concentrations for 36 h. The cell lysates were analyzed with an antibody against ARF1 or ARF6 before (total ARF1 or ARF6) and after (ARF1-GTP or ARF6-GTP) incubating with a GST-fusion protein GGA3VHS-GAT. (B) HEK293 cells were transfected with the Super(8X)TOPFlash reporter, pTK-RL plasmid, and cDNA of arfaptin 1 (red line) or ssDNA (blue line) using Fugene6. The cells were treated with QS11 at the indicated concentrations and Wnt-3a CM (1:1 vol/vol ratio to the growth medium) 24 h after transfection. Luciferase activities were measured 36 h after QS11 treatment. The activation fold was normalized against renilla luciferase. Error bars are SD. (C) The effect of QS11 on nuclear β-catenin. HEK293 cells were treated with QS11 at the indicated concentrations and Wnt-3a CM (1:1 vol/vol ratio to the growth medium) for 24 h. Nuclear β-catenin was analyzed with an antibody against β-catenin. γ-tubulin was used as the loading control.