Fig. 6.
Immunohistochemical confirmation of mRNA induction on a subset of motor neurons of both SOD1G37R and SOD1G85R mice of C1q (of the classic complement system). (A–F) Double-immunohistochemistry for C1q (red) in combination with SMI32 (to detect large neurons; green) on lumbar spinal cord sections of mutant (A and B) SOD1G37R, mutant (D and E) SOD1G85R and age-matched control WT SOD1WT (C) or nontransgenic (F) mice. C1q (a secreted polypeptide, whose mRNA was already induced at onset) was detectable at a protein level on ventral horn motor neurons of symptomatic SOD1G37R and SOD1G85R mice. No C1q could be detected on motor neurons of age-matched control mice. (A′–E′) Enlargements showing individual C1q-positive motor neurons. [Scale bar in F: 25 μm (for A–F).] [Scale bar in B′: 10 μm (for A′–E′).]