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. 2000 May 23;97(12):6499–6503. doi: 10.1073/pnas.110149597

Figure 1.

Figure 1

Specificity and duration of RNAi in S2 cell culture. (A) S2 cells were incubated with the indicated concentrations of DSH3PX1 or DACK dsRNAs. Cellular extracts were prepared after 3 days and subjected to Western analysis as described in Materials and Methods. (B) S2 cells were incubated with 37 nM DSH3PX1 dsRNA for 10–240 min. (Left) Samples undergoing the standard recovery procedure. (Right) Samples rinsed to remove the unincorporated dsRNA before addition of serum containing media. Western analysis on extracts prepared after a 3-day recovery period was performed as described using antibodies generated against DSH3PX1 and Dock. (C) S2 cells were exposed to 37 nM DSH3PX1 dsRNA as described in Materials and Methods. Cellular extracts were prepared on the indicated days and subjected to Western analysis using antibodies against DSH3PX1 and Dock. (D) The specified amounts of dsRNAs corresponding to DSH3PX1 coding sequence were added to either BG2-C6 or KC cells. Western analysis was performed as described using antibodies directed against DSH3PX1. In each panel, an arrow indicates the relative mobility of DACK, DSH3PX1, or Dock.