Fig. 1.

mtDNA mutations in primary tumor and margin tissues. (A) Frequency of mtDNA mutations in mitochondrial genes. Mutations were found in all coding genes and noncoding genes, including D-loop, 12S rRNA, 16S rRNA, and tRNA. All mutations are graphed in gray. Nonsynonymous mutations are shown in black (dAA). (B) An identical mutation in gene ND4L (G-A, at position 10695) was identified in margins with dysplasia adjacent to primary tumor 2232. Mutations were found in margin 3 (M3) having moderate and high-grade dysplasia and margin 4 (M4) having mild dysplasia. Margin 1 (M1) and margin 2 (M2) had no dysplastic changes showing no mutations. Arrows indicate mutations.