Table 1. Catalytic parameters for the enzymatic processing at 37 °C and pH 7.1 of native fibrinogen by the whole MMP-2, the catalytic domain of MMP-2 and plasmin and of peroxynitrite-treated fibrinogen by whole MMP-2.

	kcat/Km (M−1·s−1)	kcat (s−1)	Km (M)
α-Chain
Native fibrinogen
Whole MMP-2	2.29(±0.23)×105	36.4(±4.2)	1.59(±0.18)×10−4
Catalytic domain	1.09(±0.12)×104	0.19(±0.02)	1.72(±0.19)×10−5
MMP-2
Plasmin	2.04(±0.21)×105	7.7(±0.9)	3.77(±0.41)×10−5
Oxidized fibrinogen
Whole MMP-2	1.05(±0.11)×105	3.1(±0.4)	2.93(±0.35)×10−5
β-Chain
Native fibrinogen
Whole MMP-2	6.61(±0.83)×104	12.5(±1.4)	1.89(±0.20)×10−4
Catalytic domain	3.59(±0.42)×103	0.12(±0.02)	3.27(±0.41)×10−5
MMP-2
Plasmin	2.47(±0.34)×105	27.8(±2.9)	1.12(±0.15)×10−4
Oxidized fibrinogen
Whole MMP-2	1.34(±0.15)×105	1.06(±0.12)	7.93(±0.88)×10−6
γ-Chain
Native fibrinogen
Plasmin	4.96(±0.6)×104	2.35(±0.36)	4.74(±0.57)×10−5