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The mutations are listed on the right-hand side of the panels. (A) Solubility analysis of WT DCase and evolved recombinant DCases. (B) Expression test of a single mutant (Y30N), three double mutants (A18T/Y30N; A18T/K34E; and Y30N/K34E) and a triple mutant (A18T/Y30N/K34E). tot, total cell; ppt, precipitant fraction; sup, supernatant fraction.
