Table 1. Randomly evolved mutations leading to improved solubility of DCase.

Amino acid substitution	Base change (codon)	Secondary structure at position	Location	Conserved residue	Hydrophilicity change*
A18T	GCG→ACG	Turn	Surface	No	+2.5
Y30N	TAC→AAC	α-Helix	Surface	No	+2.2
K34E	AAA→GAA	α-Helix	Surface	Yes	−0.4

*Increase or decrease of hydrophilicity of the mutated sites are indicated by ‘+’, ‘−’respectively. Hydropathy indices are obtained by the method of Kyte and Doolittle [34].