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. 2007 Feb 26;402(Pt 3):591–600. doi: 10.1042/BJ20061722

Figure 2. Mutations in the insulin response element or NF-Y site reduce Fasn promoter activity.

Figure 2

Rat hepatocytes were transfected with a construct that contained the luciferase (LUC) reporter gene driven by the Fasn gene region of −150 to −43 bp fused to a generic TATA box [18]. The region contains a classical SRE, an insulin response element (IRE) at −72 to −53 bp, an Sp1 site (−91 to −81 bp), and an NF-Y site (−99 to −93 bp). Mutations (X) were introduced into the respective sites (see Table 1) as described in the Experimental section. Results are expressed relative to the wild-type sequence, and are means±S.E.M. for two or three independent hepatocyte preparations with 4–6 replications per construct within a cell preparation. *P<0.05 for the effect of the Sp1 mutation; **P<0.01 for effect of the IRE, NF-Y and NF-Y/Sp1 mutations.