Table 1.

Quantitative analysis of the repression of GFP fluorescence by RNA–protein interactions with different binding affinities

Interaction	Approx. affinity (references)*	GFP repression†
IRE/IRP-1	1.0–10 nM (21–23)	∼60–70%
U1 loop 2/U1A	0.02–80 nM (24–26)	∼40%
MSC/MS2 coat protein	0.02–0.1 nM (12, 13)	∼70%
MSA/MS2 coat protein	0.1–1 mM (13)	∼50%

^*Binding affinities for the different RNA–protein interactions as reported. 

^†GFP fluorescence of cells grown in glucose media was defined as 100%. The remaining mean fluorescence of cells after growth in galactose media was expressed as a percentage of the above, and the repression level calculated by substraction from 100%.