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. 2002 Jul 1;16(13):1682–1695. doi: 10.1101/gad.983802

Figure 1.

Figure 1

(A) Orientation and chromosome location of promoter (pGPD1-loxP) and two reporter (loxP-ura3 and loxP-ade2) constructs used in Cre/loxP assay relative to their adjacent centromeres (not drawn to scale; see text for details). (B) Temporal order of meiotic events relative to Cre-induction and return-to-growth (RTG) procedures. Timing of meiotic events is based on Padmore et al. (1991). All strains used in this study contain the ndt80Δ mutation, which blocks the exit from meiotic prophase I. The ime2Δ mutation blocks entry into meiotic DNA replication. Other mutations used in this study affect chromosomal events that occur during meiotic prophase (See text). (C, left) Commitment to prototroph formation following RTG in the presence (black symbols) or absence (open symbols) of induction of Cre-expression by the addition of galactose at t = 1 h after transfer into SPM. Data from the ndt80Δ strain designated here as “WT” are shown. Allelic interactions are reported by Ura+ prototrophs/cfu (squares), and ectopic interactions are reported by Ade+ prototrophs/cfu (circles). Right: Survivability (total cfu / cfu at t = 1 h) of ndt80Δ cells after RTG throughout the meiotic time course in the presence or absence of galactose.